Structures of the fully assembled Saccharomyces cerevisiae spliceosome before activation

The pre-catalytic spliceosome (B complex) is preceded by the pre-B complex. Here we report the cryo-EM structures of the Saccharomyces cerevisiae pre-B and B complexes at average resolutions of 3.3-4.6 and 3.9 Å, respectively. In the pre-B complex, the duplex between the 5′-splice site (5’SS) and U1 snRNA is recognized by Yhc1, Luc7, and the Sm ring. In the B complex, U1 snRNP is dissociated, the 5′-exon-5’SS sequences are translocated near U6 snRNA, and three B-specific proteins…
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